Operon Concept

IMPORTANT

Operon Concept: Overview

This topic covers concepts such as Gene Expression, Regulation of Gene Expression, Lac Operon, Repressible Operon, Tryptophan Operon, Inducible Operon, etc.

Important Questions on Operon Concept

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IMPORTANT

In lac operon, the lactose acts as

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In the lac operon shown above, name the molecule ‘X’ synthesized by ‘i’ gene and name the molecule synthesised by the Z gene:

HARD
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Proteins P1 and P2  bind to each other to form a complex. This complex has a high binding affinity to a specific element on the promoter sequence of a gene X, thus transcribing it. The product of gene Xis responsible for the accumulation of red pigment in some plants. Under drought stress, another protein P3 binds to P2 and prevents its binding to P1 and the plants appear pale. The following table depicts a representation of phenotypes of wild-type and mutant  plants.

Condition/ Plant

Wild-type

Mutant-1

Mutant-2

Mutant-3

Water sufficiency

Red

Pale

Red

Pale

Drought

Pale

Pale

Red

Pale

Choose the correct statement(s).

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In biological systems, proteins (repressors and activators) control gene expression. Repressors bind to promoters and inhibit gene expression. Some promoters require activators for gene expression. These constitute a genetic circuit and are analogous to logic gates in digital electronics. A schematic diagram of a genetic circuit in a cell is shown below. Binding of IPTG to LacI activates transcription from Plac. Binding of arabinose (Ara) to AraC activates transcription from Para.

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 The correct statement(s) regarding the given genetic circuit is/are

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For RNA polymerase, which of the following genes provides attachment site?

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Select the incorrectly matched pair of the following pairs.

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Which of the following genes provides attachment site for RNA polymerase enzyme?

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Read the following statements with respect to lac operon, and select the correct option.

A. It has three structural genes.

B. Glucose can act as an inducer.

C. Repressor protein acts in the presence of lactose.

D. Repressor is synthesized by the regulator gene.

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The regulatory proteins are the accessory proteins that interact with RNA polymerase and affect its role in transcription. Which of the following statements is true about regulatory proteins?

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You have created a fusion between trp operon and lac operon which encodes the enzymes for tryptophan biosynthesis, under the regulatory control of the lac operator. Under which of the following conditions will tryptophan synthase be induced in the strain that carries the chimeric operator fused operons?

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When does the lac operon of E. coli get switched on?

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This structural gene synthesizes the mRNA that translates the permease protein

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In an operon, a repressor is synthesised by

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In lac operon, the regulator gene is denoted as i–gene. Here the term i denotes for

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The regulation of gene expression in eukaryotes occurs at the level of :

A. Transcription level.

B. Processing level.

C. Transport of ribosomal subunits from the nucleus to the cytoplasm.

D. Translation level.

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The predominant site for the control of gene expression in prokaryote is

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The Lac operon contains three structural genes z'',y' and a'' The structural genes code for
i) The 'z' gene codes for beta-galactosidase enzyme.
ii) 'a' gene does not code for transacetylase enzyme.
iii) The 'y' gene codes for enzyme permease.
iv) 'y'' gene does not code for the enzyme permease.
v) 'a' gene codes a transacetylase enzyme.
The correct combination is

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A polycistronic structural gene is regulated by a common promoter and regulatory genes. Such arrangement is very common in . and is referred to as .

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Read the following four statements (A-D):

(A) In transcription, adenine pairs with uracil.

(B) Regulation of lac operon by repressor is referred to as positive regulation.

(C) The human genome has approximately 30,000 genes.

(D) Haemophilia is a sex-linked dominant disease.

How many of the above statements are right?

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In E.coli the lac operon gets switched on when :-